Flow Cytometry for the Diagnosis of Primary Immunodeficiency Diseases

immunoloji-17-1-2024

Recep EVCENa
aNecmettin Erbakan University Faculty of Medicine, Department of Internal Medicine, Division of Immunology and Allergy Diseases, Konya, Türkiye

Evcen R. Flow cytometry for the diagnosis of primary immunodeficiency diseases. Çölkesen F, ed. Primary Immunodeficiency Diseases in Adults. 1st ed. Ankara: Türkiye Klinikleri; 2024. p.75-82.

ABSTRACT
Flow cytometry is an indispensable tool in diagnosis of primary immunodeficiencies (PID). PIDs represent a significant health burden, associated with more than 485 different causative genes. Flow cytometry employs fluorescently labeled antibodies to measure cell size, granularity, and antigen expression levels. This article provides examples for some types of immunodeficiencies that can be diagnosed by flow cytometry. Diseases such as severe combined immunodeficiency, Bruton’s disease, chronic granulomatous disease, and common variable immunodeficiency can be diagnosed by flow cytometry. With advancing technology and discovery of new monoclonal antibodies, the role of flow cytometry in diagnosing and monitoring primary immunodeficiency is becoming more important.

Keywords: Flow cytometry; primary immunodeficiency; immunology

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